Improved methodology for efficient establishment of the myocardial ischemia-reperfusion model in pigs through the median thoracic incision

Liuhua Zhou; Jiateng Sun; Tongtong Yang; Sibo Wang; Tiankai Shan; Lingfeng Gu; Jiawen Chen; Tianwen Wei; Di Zhao; Chong Du; Yulin Bao; Hao Wang; Xiaohu Lu; Haoliang Sun; Meng Lv; Di Yang; Liansheng Wang

doi:10.7555/JBR.36.20220189

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Liuhua Zhou, Jiateng Sun, Tongtong Yang, Sibo Wang, Tiankai Shan, Lingfeng Gu, Jiawen Chen, Tianwen Wei, Di Zhao, Chong Du, Yulin Bao, Hao Wang, Xiaohu Lu, Haoliang Sun, Meng Lv, Di Yang, Liansheng Wang. Improved methodology for efficient establishment of the myocardial ischemia-reperfusion model in pigs through the median thoracic incision[J]. The Journal of Biomedical Research. doi: 10.7555/JBR.36.20220189

Citation:

Liuhua Zhou, Jiateng Sun, Tongtong Yang, Sibo Wang, Tiankai Shan, Lingfeng Gu, Jiawen Chen, Tianwen Wei, Di Zhao, Chong Du, Yulin Bao, Hao Wang, Xiaohu Lu, Haoliang Sun, Meng Lv, Di Yang, Liansheng Wang. Improved methodology for efficient establishment of the myocardial ischemia-reperfusion model in pigs through the median thoracic incision[J]. The Journal of Biomedical Research. doi: 10.7555/JBR.36.20220189

Citation:

Liuhua Zhou, Jiateng Sun, Tongtong Yang, Sibo Wang, Tiankai Shan, Lingfeng Gu, Jiawen Chen, Tianwen Wei, Di Zhao, Chong Du, Yulin Bao, Hao Wang, Xiaohu Lu, Haoliang Sun, Meng Lv, Di Yang, Liansheng Wang. Improved methodology for efficient establishment of the myocardial ischemia-reperfusion model in pigs through the median thoracic incision[J]. The Journal of Biomedical Research. doi: 10.7555/JBR.36.20220189

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Improved methodology for efficient establishment of the myocardial ischemia-reperfusion model in pigs through the median thoracic incision

doi: 10.7555/JBR.36.20220189

Liuhua Zhou^{1, △},
Jiateng Sun^{1, △},
Tongtong Yang^{1, △},
Sibo Wang¹,
Tiankai Shan¹,
Lingfeng Gu¹,
Jiawen Chen¹,
Tianwen Wei¹,
Di Zhao¹,
Chong Du¹,
Yulin Bao¹,
Hao Wang¹,
Xiaohu Lu²,
Haoliang Sun²,
Meng Lv³,
Di Yang^{1
,
,},
Liansheng Wang^{1
,
,}

1.
Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China
2.
Department of Cardiac surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China
3.
Medical Experimental Animal Center, Nanjing Medical University, Nanjing, Jiangsu 210029, China

More Information

Corresponding author: Liansheng Wang and Di Yang, Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu 210029, China. Tel/Fax: +86-25-83724440 and +86-25-68136721, E-mails: drlswang@njmu.edu.cn and diyang@njmu.edu.cn;
Received: 2022-08-24
Revised: 2022-10-08
Accepted: 2022-10-16

Published: 2023-01-20

Abstract

Abstract

To investigate the feasibility and effectiveness of establishing porcine ischemia-reperfusion models by ligating the left anterior descending (LAD) coronary artery, we first randomly divided 16 male Bama pigs into a sham group and a model group. After anesthesia, we separated the arteries and veins. Subsequently, we rapidly located the LAD coronary artery at the beginning of its first diagonal branch through a mid-chest incision. Then, we loosened and released the ligation line after five minutes of pre-occlusion. Finally, we ligated the LAD coronary artery in situ two minutes later and loosened the ligature 60 min after ischemia. Compared with the sham group, electrocardiogram showed multiple continuous lead ST-segment elevations, and ultrasound cardiogram showed significantly lower ejection fraction and left ventricular fractional shortening at one hour and seven days post-operation in the model group. Twenty-four hours after the operation, cardiac troponin T and creatine kinase-MB isoenzyme levels significantly increased in the model group, compared with the sham group. Hematoxylin and eosin staining showed the presence of many inflammatory cells infiltrating the interstitium of the myocardium in the model group but not in the sham group. Masson staining revealed a significant increase in infarct size in the ischemia/reperfusion group. All eight pigs in the model group recovered with normal sinus heart rates, and the survival rate was 100%. In conclusion, the method can provide an accurate and stable large animal model for preclinical research on ischemia/reperfusion with a high success rate and homogeneity of the myocardial infarction area.
- coronary artery ligation,
- myocardial ischemia-reperfusion injury,
- Bama pig,
- animal model

CLC number: R542.2, Document code: A
The authors reported no conflict of interests.
△These authors contributed equally to this work.

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References(32)

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