A visualization pipeline for <i>in vivo</i> two-photon volumetric astrocytic calcium imaging

Qian Sun; Yusi Hu; Saiyue Deng; Yanyu Xiong; Zhili Huang

doi:10.7555/JBR.36.20220099

Volume 36 Issue 5

Sep. 2022

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Article Navigation > The Journal of Biomedical Research > 2022 > 36(5): 358-367

Qian Sun, Yusi Hu, Saiyue Deng, Yanyu Xiong, Zhili Huang. A visualization pipeline for in vivo two-photon volumetric astrocytic calcium imaging[J]. The Journal of Biomedical Research, 2022, 36(5): 358-367. doi: 10.7555/JBR.36.20220099

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Qian Sun, Yusi Hu, Saiyue Deng, Yanyu Xiong, Zhili Huang. A visualization pipeline for in vivo two-photon volumetric astrocytic calcium imaging[J]. The Journal of Biomedical Research, 2022, 36(5): 358-367. doi: 10.7555/JBR.36.20220099

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A visualization pipeline for in vivo two-photon volumetric astrocytic calcium imaging

doi: 10.7555/JBR.36.20220099

Qian Sun^{1, △
,},
Yusi Hu^{1, 2, △
,},
Saiyue Deng³,
Yanyu Xiong^{1, 2},
Zhili Huang^{1, 2
,
,}

1.
Department of Pharmacology, School of Basic Medical Science, Fudan University, Shanghai 200032, China
2.
State Key Laboratory of Medical Neurobiology, Institutes of Brain Science and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, China
3.
Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China

Funds: This study was supported in part by Shanghai Committee of Science and Technology (Grant No. 20ZR1403500) and the Shanghai Medical Research Council. The cartoon image was obtained from Scidraw.io.

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Corresponding author: Zhili Huang, Department of Pharmacology, School of Basic Medical Science, Fudan University, 130 Dong'an Road, Shanghai 200032, China. Tel/Fax: +86-21-54237043/+86-21-54237103, E-mail: huangzl@fudan.edu.cn
Received: 2022-05-01
Revised: 2022-06-19
Accepted: 2022-07-11

Published: 2022-08-10

Issue Date: 2022-09-28

Abstract

Abstract

Astrocytes, the multi-functional glial cells with the most abundant population in the brain, integrate information across their territories to regulate neuronal synaptic and cerebrovascular activities. Astrocytic calcium (Ca²⁺) signaling is the major readout of cellular functional state of astrocytes. The conventional two-photon in vivo imaging usually focuses on a single horizontal focal plane to capture the astrocytic Ca²⁺ signals, which leaves >80% spatial information undetected. To fully probe the Ca²⁺ activity across the whole astrocytic territory, we developed a pipeline for imaging and visualizing volumetric astrocytic Ca²⁺ time-lapse images. With the pipeline, we discovered a new signal distribution pattern from three-dimensional (3D) astrocytic Ca²⁺ imaging data of mice under isoflurane anesthetic states. The tools developed in this study enable a better understanding of the spatiotemporal patterns of astrocytic activity in 3D space.
- astrocyte,
- calcium imaging,
- three-dimensional visualization

CLC number: R338.2, Document code: A
The authors reported no conflict of interests.
^△These authors contributed equally to this work.

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References(40)

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