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  • ISSN 1674-8301
  • CN 32-1810/R
Volume 36 Issue 5
Sep.  2022
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Qian Sun, Yusi Hu, Saiyue Deng, Yanyu Xiong, Zhili Huang. A visualization pipeline for in vivo two-photon volumetric astrocytic calcium imaging[J]. The Journal of Biomedical Research, 2022, 36(5): 358-367. doi: 10.7555/JBR.36.20220099
Citation: Qian Sun, Yusi Hu, Saiyue Deng, Yanyu Xiong, Zhili Huang. A visualization pipeline for in vivo two-photon volumetric astrocytic calcium imaging[J]. The Journal of Biomedical Research, 2022, 36(5): 358-367. doi: 10.7555/JBR.36.20220099

A visualization pipeline for in vivo two-photon volumetric astrocytic calcium imaging

doi: 10.7555/JBR.36.20220099
Funds:  This study was supported in part by Shanghai Committee of Science and Technology (Grant No. 20ZR1403500) and the Shanghai Medical Research Council. The cartoon image was obtained from Scidraw.io.
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  • Corresponding author: Zhili Huang, Department of Pharmacology, School of Basic Medical Science, Fudan University, 130 Dong'an Road, Shanghai 200032, China. Tel/Fax: +86-21-54237043/+86-21-54237103, E-mail: huangzl@fudan.edu.cn
  • Received: 2022-05-01
  • Revised: 2022-06-19
  • Accepted: 2022-07-11
  • Published: 2022-08-10
  • Issue Date: 2022-09-28
  • Astrocytes, the multi-functional glial cells with the most abundant population in the brain, integrate information across their territories to regulate neuronal synaptic and cerebrovascular activities. Astrocytic calcium (Ca2+) signaling is the major readout of cellular functional state of astrocytes. The conventional two-photon in vivo imaging usually focuses on a single horizontal focal plane to capture the astrocytic Ca2+ signals, which leaves >80% spatial information undetected. To fully probe the Ca2+ activity across the whole astrocytic territory, we developed a pipeline for imaging and visualizing volumetric astrocytic Ca2+ time-lapse images. With the pipeline, we discovered a new signal distribution pattern from three-dimensional (3D) astrocytic Ca2+ imaging data of mice under isoflurane anesthetic states. The tools developed in this study enable a better understanding of the spatiotemporal patterns of astrocytic activity in 3D space.


  • CLC number: R338.2, Document code: A
    The authors reported no conflict of interests.
    These authors contributed equally to this work.
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