• ISSN 1674-8301
  • CN 32-1810/R
Volume 31 Issue 5
Sep.  2017
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Article Contents
Young-Joo Yi, S. Kamala-Kannan, Jeong-Muk Lim, Byung-Taek Oh, Sang-Myeong Lee. Effects of difructose dianhydride (DFA)-IV on in vitro fertilizationin pigs[J]. The Journal of Biomedical Research, 2017, 31(5): 453-461. doi: 10.7555/JBR.31.20160115
Citation: Young-Joo Yi, S. Kamala-Kannan, Jeong-Muk Lim, Byung-Taek Oh, Sang-Myeong Lee. Effects of difructose dianhydride (DFA)-IV on in vitro fertilization in pigs[J]. The Journal of Biomedical Research, 2017, 31(5): 453-461. doi: 10.7555/JBR.31.20160115

Effects of difructose dianhydride (DFA)-IV on in vitro fertilization in pigs

doi: 10.7555/JBR.31.20160115
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We thank Hee-Jung Lee for assistance with the experiments, and Agency for Korea National Food Cluster for HPLC analysis. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2013R1A6A3A- 04063769)

  • Received: 2016-09-12
  • Revised: 2017-11-24
  • Issue Date: 2017-09-22
  • Difructose dianhydride IV (DFA-IV) is produced from levan, which is a natural polysaccharide that belongs to the fructan family, through the activity of levan fructotransferase (LF) derived from microorganisms. Recently, DFA-IV has been expected to have diverse applications in the food and medical industry. Here, we examined the potential application of DFA-IV for in vitro fertilization (IVF) in pigs. In the assessment of acrosomal integrity during incubation, intact acrosomal or viable spermatozoa were highly sustained in 0.1% or 0.25% DFA-IV (69.8%-70.8%, P < 0.05). Reactive oxygen species (ROS) levels during sperm incubation decreased following the addition of DFAIV, and 0.1%-0.5% DFA-IV in particular significantly decreased ROS production relative to that seen with no addition or 0.75% DFA-IV. Total fertilization (mono + polyspermic oocyte) rate was significantly higher in the addition of 0.1% DFA-IV (94.2%) than with other concentrations (71.8%-86.7%, P < 0.05). When using reduced IVF times and lower sperm numbers, we found that addition of 0.1%–0.5% DFA-IV significantly increased the fertilization rate (P < 0.05). Fertilized oocytes treated with 0.1% DFA-IV exhibited higher embryonic development and blastocyst formation than those treated with other concentrations (P < 0.05). Consequently, the addition of DFA-IV during IVF improved fertilization and embryonic development, suggesting the possible use of novel sugars for enhancement of assisted reproductive technology (ART) in mammals.

     

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