• ISSN 1674-8301
  • CN 32-1810/R
Volume 25 Issue 6
Nov.  2011
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Hong Yan, Dong Kong, Xiaomei Ge, Xiang Gao, Xiao Han. Generation of conditional knockout alleles for PRL-3[J]. The Journal of Biomedical Research, 2011, 25(6): 438-443. doi: 10.1016/S1674-8301(11)60058-4
Citation: Hong Yan, Dong Kong, Xiaomei Ge, Xiang Gao, Xiao Han. Generation of conditional knockout alleles for PRL-3[J]. The Journal of Biomedical Research, 2011, 25(6): 438-443. doi: 10.1016/S1674-8301(11)60058-4

Generation of conditional knockout alleles for PRL-3

doi: 10.1016/S1674-8301(11)60058-4
Funds:

This work was supported by Grants from MOST of China (No. 2005CB522501, No.2006BAI23B00, and No.2006CB943503) to Xiang Gao.

  • Received Date: 2011-06-11
  • Phosphatase of regenerating liver-3 (PRL-3) is a member of the protein tyrosine phosphatase (PTP) superfamily and is highly expressed in cancer metastases. For better understanding of the role of PRL-3 in tumor metastasis, we applied a rapid and efficient method for generating PRL-3 floxed mice and investigated its phenotypes. A BAC retrieval strategy was applied to construct the PRL-3 conditional gene-targeting vector. Exon 4 was selected for deletion to generate a nonfunctional prematurely terminated short peptide as it will cause a frame-shift mutation. Conditional knockout PRL-3 mice were generated by using the Cre-loxP system and were validated by Southern blot and RT-PCR analysis. Further analysis revealed the phenotype characteristics of PRL-3 knockout mice and wildtype mice. In this study, we successfully constructed the PRL-3 conditional knockout mice, which will be helpful to clarify the roles of PRL-3 and the mechanisms in tumor metastasis.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Generation of conditional knockout alleles for PRL-3

doi: 10.1016/S1674-8301(11)60058-4
Funds:

This work was supported by Grants from MOST of China (No. 2005CB522501, No.2006BAI23B00, and No.2006CB943503) to Xiang Gao.

Abstract: Phosphatase of regenerating liver-3 (PRL-3) is a member of the protein tyrosine phosphatase (PTP) superfamily and is highly expressed in cancer metastases. For better understanding of the role of PRL-3 in tumor metastasis, we applied a rapid and efficient method for generating PRL-3 floxed mice and investigated its phenotypes. A BAC retrieval strategy was applied to construct the PRL-3 conditional gene-targeting vector. Exon 4 was selected for deletion to generate a nonfunctional prematurely terminated short peptide as it will cause a frame-shift mutation. Conditional knockout PRL-3 mice were generated by using the Cre-loxP system and were validated by Southern blot and RT-PCR analysis. Further analysis revealed the phenotype characteristics of PRL-3 knockout mice and wildtype mice. In this study, we successfully constructed the PRL-3 conditional knockout mice, which will be helpful to clarify the roles of PRL-3 and the mechanisms in tumor metastasis.

Hong Yan, Dong Kong, Xiaomei Ge, Xiang Gao, Xiao Han. Generation of conditional knockout alleles for PRL-3[J]. The Journal of Biomedical Research, 2011, 25(6): 438-443. doi: 10.1016/S1674-8301(11)60058-4
Citation: Hong Yan, Dong Kong, Xiaomei Ge, Xiang Gao, Xiao Han. Generation of conditional knockout alleles for PRL-3[J]. The Journal of Biomedical Research, 2011, 25(6): 438-443. doi: 10.1016/S1674-8301(11)60058-4

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