• ISSN 1674-8301
  • CN 32-1810/R
Volume 25 Issue 2
Mar.  2011
Turn off MathJax
Article Contents

Wei Li, Wu Di, Lijuan Hua, Bingrong Zhou, Ze Guo, Dan Luo. UVB suppresses PTEN expression by upregulating miR-141 in HaCaT cells[J]. The Journal of Biomedical Research, 2011, 25(2): 135-140. doi: 10.1016/S1674-8301(11)60017-1
Citation: Wei Li, Wu Di, Lijuan Hua, Bingrong Zhou, Ze Guo, Dan Luo. UVB suppresses PTEN expression by upregulating miR-141 in HaCaT cells[J]. The Journal of Biomedical Research, 2011, 25(2): 135-140. doi: 10.1016/S1674-8301(11)60017-1

UVB suppresses PTEN expression by upregulating miR-141 in HaCaT cells

doi: 10.1016/S1674-8301(11)60017-1
Funds:

This study was supported by the National Natural Science Foundation of China (No. 30771946 and No. 81000700).

  • MicroRNAs (miRNAs) are 21 to 24 nucleotide, non-coding RNA molecules that post-transcriptionally regulate the expression of target genes. Ultraviolet B (UVB) radiation has been shown to inhibit phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression in HaCaT cells through an unknown mechanism. In this study, we investigated whether miR-141 can regulate UVB exposure-mediated inhibition of PTEN expression. Real-time RT-PCR, annexin V/fluorescein isothiocyanate staining, Western blotting and anti-miRNA oligonucle-otide transfection were employed in this study. We found that upregulation of miR-141 expression after UVB ir-radiation was inversely correlated with PTEN expression levels in HaCaT cells. Furthermore, miR-141 expression increased apoptosis, while anti-miR-141 partly restored PTEN expression and reversed the pro-apoptosis effect of UVB. UVB suppresses the expression of PTEN by upregulating miR-141 in HaCaT cells. Therefore, miR-141 is a potential gene therapy target for UVB-induced photodamage.
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(4390) PDF downloads(1352) Cited by()

Related
Proportional views

UVB suppresses PTEN expression by upregulating miR-141 in HaCaT cells

doi: 10.1016/S1674-8301(11)60017-1
Funds:

This study was supported by the National Natural Science Foundation of China (No. 30771946 and No. 81000700).

Abstract: MicroRNAs (miRNAs) are 21 to 24 nucleotide, non-coding RNA molecules that post-transcriptionally regulate the expression of target genes. Ultraviolet B (UVB) radiation has been shown to inhibit phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression in HaCaT cells through an unknown mechanism. In this study, we investigated whether miR-141 can regulate UVB exposure-mediated inhibition of PTEN expression. Real-time RT-PCR, annexin V/fluorescein isothiocyanate staining, Western blotting and anti-miRNA oligonucle-otide transfection were employed in this study. We found that upregulation of miR-141 expression after UVB ir-radiation was inversely correlated with PTEN expression levels in HaCaT cells. Furthermore, miR-141 expression increased apoptosis, while anti-miR-141 partly restored PTEN expression and reversed the pro-apoptosis effect of UVB. UVB suppresses the expression of PTEN by upregulating miR-141 in HaCaT cells. Therefore, miR-141 is a potential gene therapy target for UVB-induced photodamage.

Wei Li, Wu Di, Lijuan Hua, Bingrong Zhou, Ze Guo, Dan Luo. UVB suppresses PTEN expression by upregulating miR-141 in HaCaT cells[J]. The Journal of Biomedical Research, 2011, 25(2): 135-140. doi: 10.1016/S1674-8301(11)60017-1
Citation: Wei Li, Wu Di, Lijuan Hua, Bingrong Zhou, Ze Guo, Dan Luo. UVB suppresses PTEN expression by upregulating miR-141 in HaCaT cells[J]. The Journal of Biomedical Research, 2011, 25(2): 135-140. doi: 10.1016/S1674-8301(11)60017-1

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return