Improved methodology for efficient establishment of the myocardial ischemia-reperfusion model in pigs through the median thoracic incision

Liu-Hua Zhou; Jia-Teng Sun; Tong-Tong Yang; Si-Bo Wang; Tian-Kai Shan; Ling-Feng Gu; Jia-Wen Chen; Tian-Wen Wei; Di Zhao; Chong Du; Yu-Lin Bao; Hao Wang; Xiao-Hu Lu; Hao-Liang Sun; Meng Lv; Di Yang; Lian-Sheng Wang

doi:10.7555/JBR.36.20220189

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Liu-Hua Zhou, Jia-Teng Sun, Tong-Tong Yang, Si-Bo Wang, Tian-Kai Shan, Ling-Feng Gu, Jia-Wen Chen, Tian-Wen Wei, Di Zhao, Chong Du, Yu-Lin Bao, Hao Wang, Xiao-Hu Lu, Hao-Liang Sun, Meng Lv, Di Yang, Lian-Sheng Wang. Improved methodology for efficient establishment of the myocardial ischemia-reperfusion model in pigs through the median thoracic incision[J]. The Journal of Biomedical Research. doi: 10.7555/JBR.36.20220189

Citation:

Liu-Hua Zhou, Jia-Teng Sun, Tong-Tong Yang, Si-Bo Wang, Tian-Kai Shan, Ling-Feng Gu, Jia-Wen Chen, Tian-Wen Wei, Di Zhao, Chong Du, Yu-Lin Bao, Hao Wang, Xiao-Hu Lu, Hao-Liang Sun, Meng Lv, Di Yang, Lian-Sheng Wang. Improved methodology for efficient establishment of the myocardial ischemia-reperfusion model in pigs through the median thoracic incision[J]. The Journal of Biomedical Research. doi: 10.7555/JBR.36.20220189

Citation:

Liu-Hua Zhou, Jia-Teng Sun, Tong-Tong Yang, Si-Bo Wang, Tian-Kai Shan, Ling-Feng Gu, Jia-Wen Chen, Tian-Wen Wei, Di Zhao, Chong Du, Yu-Lin Bao, Hao Wang, Xiao-Hu Lu, Hao-Liang Sun, Meng Lv, Di Yang, Lian-Sheng Wang. Improved methodology for efficient establishment of the myocardial ischemia-reperfusion model in pigs through the median thoracic incision[J]. The Journal of Biomedical Research. doi: 10.7555/JBR.36.20220189

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Improved methodology for efficient establishment of the myocardial ischemia-reperfusion model in pigs through the median thoracic incision

doi: 10.7555/JBR.36.20220189

1.
Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China
2.
Department of Cardiac surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China
3.
Medical Experimental Animal Center, Nanjing Medical University, Nanjing, Jiangsu 210029, China

More Information

Corresponding author: Lian-Sheng Wang and Di Yang, Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu 210029, China. Tel/Fax: +86-25-83724440, E-mails: drlswang@njmu.edu.cn and ***;
Received: 2022-08-24
Revised: 2022-10-08
Accepted: 2022-10-16

Published: 2023-01-20

Abstract

Abstract

To investigate feasibility and effectiveness of the establishing porcine ischemia-reperfusion models by ligating the left anterior descending (LAD) coronary artery, we first randomly divided 16 male Bama pigs into a sham group and a model group. After anesthesia, we separated the arteries and veins and passed the LAD coronary artery through a median thoracic incision to be below the beginning of its first diagonal branch. Then, we loosened and released the ligation line after five minutes of pre-occlusion. Finally, we ligated the LAD coronary artery in situ two minutes later and loosened the ligature 60 minutes after ischemia. Compared with the sham group, ECG showed that ST-segment was ?non-significantly? elevated in multiple continuous leads, and UCG showed that EF and FS were significantly lower at one hour and seven days after operation in the model group. Twenty four hours after operation, the cTnT and CK-MB levels significantly increased in the model group than that in the sham group. The hematoxylin and eosin staining showed many inflammatory cells infiltrating the interstitium of the myocardium in the model group but not in the sham group. Masson staining showed a significant increase in infarct size in the ischemia/reperfusion group. All eight pigs in the model group recovered with normal sinus heart rates, and the survival rate was 100%. In conclusion, the method can provide an accurate and stable large animal model for preclinical research of ischemia/reperfusion with a high success rate and homogeneity of the myocardial infarction area.
- coronary artery ligation,
- myocardial ischemia-reperfusion injury,
- Bama pig,
- animal model

CLC number: R542.2, Document code: A
The authors reported no conflict of interests.
△These authors contributed equally to this work.

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References(32)

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