• ISSN 1674-8301
  • CN 32-1810/R
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Gelena Valerievna Kakurina, Marina Stakheeva, Elena Sereda, Evgenia Sidenko, Olga Cheremisina, Evgeny Choinzonov, Irina Kondakova. Pilot study of the relative number of circulating tumor cells and leukocytes containing actin-binding proteins in head and neck cancer patients[J]. The Journal of Biomedical Research. doi: 10.7555/JBR.36.20220182
Citation: Gelena Valerievna Kakurina, Marina Stakheeva, Elena Sereda, Evgenia Sidenko, Olga Cheremisina, Evgeny Choinzonov, Irina Kondakova. Pilot study of the relative number of circulating tumor cells and leukocytes containing actin-binding proteins in head and neck cancer patients[J]. The Journal of Biomedical Research. doi: 10.7555/JBR.36.20220182

Pilot study of the relative number of circulating tumor cells and leukocytes containing actin-binding proteins in head and neck cancer patients

doi: 10.7555/JBR.36.20220182
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  • Corresponding author: Gelena Valerievna Kakurina, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 5 Kooperativny Street, Tomsk 634009, Russia. Tel/Fax: +7-3822-51-33-06/+7-3822-51-33-06, E-mail: kakurinagv@oncology.tomsk.ru/kakurina.gv@ssmu.ru
  • Received: 2022-04-15
  • Revised: 2022-08-17
  • Accepted: 2022-08-30
  • Published: 2022-11-10
  • Circulating tumor cells (CTCs) play an important role in tumor metastases, which positively correlates with an increased risk of death. Actin-binding proteins, i.e., cofilin (CFL1), profilin 1 (PFN1), and adenylate cyclase-associated protein 1 (CAP1) are thought to be involved in tumor cell motility and metastasis, specifically in head and neck squamous cell carcinoma (HNSCC). However, there are currently no published studies of CFL1, PFN1, and CAP1 in CTCs and leukocytes in HNSCC patients. We assessed serum levels of CFL1, PFN1, and CAP1 and the number of CTCs and leukocytes containing these proteins in blood from 31 HNSCC patients (T1–4N0–2M0). Analysis was conducted using flow cytometry and an ELISA kit. We found that CAP1+ CTCs and CAP1+ leukocyte subgroups were prevalent in our HNSCC patient samples, while the prevalence rates of CFL1+ and PFN1+ CTCs was relatively low. Patients with stage T2–4N1–2M0 had CFL1+ and PFN1+ CTCs, and with an elevated PFN1 serum level. In general, PFN1 serum level and the relative number of PFN1+CD326+ CTCs could be valuable prognostic markers for HNSCC metastases. This study is the first to obtain data regarding the contents of ABPs in CTCs, and leukocytes in blood from HNSCC patients. This is also the first to assess the relationship between the number of CTCs subgroups and disease characteristics.


  • CLC number: R739.91, Document code: A
    The authors reported no conflict of interests.
    ΔThese authors contributed equally to this work.
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