• ISSN 1674-8301
  • CN 32-1810/R
Volume 37 Issue 2
Mar.  2023
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Article Contents
Wan Jiang, Jiajia Chen, Olivia Ewi Vidjro, Yingying Zhang, Gengni Guo, Ziyi Li, Yize Qi, Rouli Dai, Tengfei Ma. Construction and evaluation of an alcohol vapor chamber system[J]. The Journal of Biomedical Research, 2023, 37(2): 115-124. doi: 10.7555/JBR.36.20220151
Citation: Wan Jiang, Jiajia Chen, Olivia Ewi Vidjro, Yingying Zhang, Gengni Guo, Ziyi Li, Yize Qi, Rouli Dai, Tengfei Ma. Construction and evaluation of an alcohol vapor chamber system[J]. The Journal of Biomedical Research, 2023, 37(2): 115-124. doi: 10.7555/JBR.36.20220151

Construction and evaluation of an alcohol vapor chamber system

doi: 10.7555/JBR.36.20220151
Funds:  This research was supported by the National Natural Science Foundation of China (Grant No. 81971246) and College Students' innovation and entrepreneurship training program in Jiangsu Province (Grant No. 202010312042Y).
More Information
  • Corresponding author: Rouli Dai, National Institute of Drug Clinical Trial, The Fourth Affiliated Hospital of Nanjing Medical University, 298 Nanpu Road, Jiangbei New District, Nanjing, Jiangsu 210031, China. Tel: +86-25-56681087, E-mail: dairouli@163.com; Tengfei Ma, School of Pharmacy, Translational Medicine Research Center for Drug Dependence and Withdrawal, Nanjing Medical University, 101 Longmian Avenue, Jiangning District, Nanjing, Jiangsu 211166, China. Tel: +86-25-86868467, E-mail: tfma@njmu.edu.cn
  • Received: 2022-06-25
  • Revised: 2022-08-29
  • Accepted: 2022-09-04
  • Published: 2022-10-28
  • Issue Date: 2023-03-28
  • An increasing number of studies demonstrated that alcohol vapor chamber is an effective way to model physical signs of alcohol use disorders. Although researchers are developing different vapor chambers to study chronic alcohol exposure model worldwide, few studies build and modify their own vapor chambers in China. Here, we designed and established an alcohol vapor chamber system for small animals. We described a paradigm showing how to control and monitor alcohol concentration in whole system. The vapor chamber system with several advantages including accommodating up to ten standard mouse cages. Furthermore, the system was tested by evaluating the blood alcohol concentration and neuron injury in mice. Importantly, the alcohol withdrawal after vapor exposure caused motor coordination impairment, anxiolytic- and depression-like behavior. Finally, the N-methyl-D-aspartate receptor (NMDAR)-mediated glutamatergic transmissions in the medial prefrontal cortex was changed after alcohol vapor exposure-induced behaviors. The frequency and amplitude of spontaneous excitatory postsynaptic currents between control and alcohol groups were not different, suggesting that alcohol exposure-induced behaviors are associated with the change in NMDAR response. Taken together, the new alcohol vapor chamber system was constructed, which would help to research the relationship between the stable alcohol exposure and withdrawal behaviors and to study chronic alcohol exposure-induced disorders in China.


  • CLC number: R749.6, Document code: A
    The authors reported no conflict of interests.
    These authors contributed equally to this work.
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