Construction and evaluation of an alcohol vapor chamber system

Wan Jiang; Jiajia Chen; Olivia Ewi Vidjro; Yingying Zhang; Gengni Guo; Ziyi Li; Yize Qi; Rouli Dai; Tengfei Ma

doi:10.7555/JBR.36.20220151

Volume 37 Issue 2

Mar. 2023

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Article Navigation > The Journal of Biomedical Research > 2023 > 37(2): 115-124

Wan Jiang, Jiajia Chen, Olivia Ewi Vidjro, Yingying Zhang, Gengni Guo, Ziyi Li, Yize Qi, Rouli Dai, Tengfei Ma. Construction and evaluation of an alcohol vapor chamber system[J]. The Journal of Biomedical Research, 2023, 37(2): 115-124. doi: 10.7555/JBR.36.20220151

Citation:

Wan Jiang, Jiajia Chen, Olivia Ewi Vidjro, Yingying Zhang, Gengni Guo, Ziyi Li, Yize Qi, Rouli Dai, Tengfei Ma. Construction and evaluation of an alcohol vapor chamber system[J]. The Journal of Biomedical Research, 2023, 37(2): 115-124. doi: 10.7555/JBR.36.20220151

Citation:

Wan Jiang, Jiajia Chen, Olivia Ewi Vidjro, Yingying Zhang, Gengni Guo, Ziyi Li, Yize Qi, Rouli Dai, Tengfei Ma. Construction and evaluation of an alcohol vapor chamber system[J]. The Journal of Biomedical Research, 2023, 37(2): 115-124. doi: 10.7555/JBR.36.20220151

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Construction and evaluation of an alcohol vapor chamber system

doi: 10.7555/JBR.36.20220151

Wan Jiang^{1, △},
Jiajia Chen^{1, △},
Olivia Ewi Vidjro^{2, 3},
Yingying Zhang¹,
Gengni Guo²,
Ziyi Li^{2, 3},
Yize Qi^{2, 3},
Rouli Dai^{4
,
,},
Tengfei Ma^{2, 3
,
,}

1.
Grade 2018, School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu 211166, China
2.
School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu 211166, China
3.
Translational Medicine Research Center for Drug Dependence and Withdrawal, Nanjing Medical University, Jiangsu 211166, China
4.
National Institute of Drug Clinical Trial, The Fourth Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210031, China

Funds: This research was supported by the National Natural Science Foundation of China (Grant No. 81971246) and College Students' innovation and entrepreneurship training program in Jiangsu Province (Grant No. 202010312042Y).

More Information

Corresponding author: Rouli Dai, National Institute of Drug Clinical Trial, The Fourth Affiliated Hospital of Nanjing Medical University, 298 Nanpu Road, Jiangbei New District, Nanjing, Jiangsu 210031, China. Tel: +86-25-56681087, E-mail: dairouli@163.com; Tengfei Ma, School of Pharmacy, Translational Medicine Research Center for Drug Dependence and Withdrawal, Nanjing Medical University, 101 Longmian Avenue, Jiangning District, Nanjing, Jiangsu 211166, China. Tel: +86-25-86868467, E-mail: tfma@njmu.edu.cn
Received: 2022-06-25
Revised: 2022-08-29
Accepted: 2022-09-04

Published: 2022-10-28

Issue Date: 2023-03-28

Abstract

Abstract

An increasing number of studies demonstrated that alcohol vapor chamber is an effective way to model physical signs of alcohol use disorders. Although researchers are developing different vapor chambers to study chronic alcohol exposure model worldwide, few studies build and modify their own vapor chambers in China. Here, we designed and established an alcohol vapor chamber system for small animals. We described a paradigm showing how to control and monitor alcohol concentration in whole system. The vapor chamber system with several advantages including accommodating up to ten standard mouse cages. Furthermore, the system was tested by evaluating the blood alcohol concentration and neuron injury in mice. Importantly, the alcohol withdrawal after vapor exposure caused motor coordination impairment, anxiolytic- and depression-like behavior. Finally, the N-methyl-D-aspartate receptor (NMDAR)-mediated glutamatergic transmissions in the medial prefrontal cortex was changed after alcohol vapor exposure-induced behaviors. The frequency and amplitude of spontaneous excitatory postsynaptic currents between control and alcohol groups were not different, suggesting that alcohol exposure-induced behaviors are associated with the change in NMDAR response. Taken together, the new alcohol vapor chamber system was constructed, which would help to research the relationship between the stable alcohol exposure and withdrawal behaviors and to study chronic alcohol exposure-induced disorders in China.
- alcohol use disorders,
- alcohol vapor model,
- anxiety,
- depression,
- NMDA receptor

CLC number: R749.6, Document code: A
The authors reported no conflict of interests.
^△These authors contributed equally to this work.

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References(31)

References

[1]	Koob GF, Volkow ND. Neurobiology of addiction: a neurocircuitry analysis[J]. Lancet Psychiatry, 2016, 3(8): 760–773. doi: 10.1016/S2215-0366(16)00104-8
[2]	Heilig M, Goldman D, Berrettini W, et al. Pharmacogenetic approaches to the treatment of alcohol addiction[J]. Nat Rev Neurosci, 2011, 12(11): 670–84. doi: 10.1038/nrn3110
[3]	Xiao HW, Ge C, Feng GX, et al. Gut microbiota modulates alcohol withdrawal-induced anxiety in mice[J]. Toxicol Lett, 2018, 287: 23–30. doi: 10.1016/j.toxlet.2018.01.021
[4]	Mereish EH, Kuerbis A, Morgenstern J. A daily diary study of stressful and positive events, alcohol use, and addiction severity among heavy drinking sexual minority men[J]. Drug Alcohol Depend, 2018, 187: 149–154. doi: 10.1016/j.drugalcdep.2018.03.003
[5]	Fu R, Tang Y, Li W, et al. Endocannabinoid signaling in the lateral habenula regulates pain and alcohol consumption[J]. Transl Psychiatry, 2021, 11(1): 220. doi: 10.1038/s41398-021-01337-3
[6]	Jeanblanc J, Rolland B, Gierski F, et al. Animal models of binge drinking, current challenges to improve face validity[J]. Neurosci Biobehav Rev, 2019, 106: 112–121. doi: 10.1016/j.neubiorev.2018.05.002
[7]	Altshuler HL. Animal models for alcohol research[J]. Curr Alcohol, 1981, 8: 343–57. https://pubmed.ncbi.nlm.nih.gov/6806018/
[8]	Gozzi A, Agosta F, Massi M, et al. Reduced limbic metabolism and fronto-cortical volume in rats vulnerable to alcohol addiction[J]. Neuroimage, 2013, 69: 112–119. doi: 10.1016/j.neuroimage.2012.12.015
[9]	Zhou L, Xie Q, Zhang Q, et al. Dexmedetomidine attenuates motor deficits via restoring the function of neurons in the nigrostriatal circuit in Parkinson's disease model mice[J]. Eur J Pharmacol, 2022, 920: 174806. doi: 10.1016/j.ejphar.2022.174806
[10]	Likhtik E, Stujenske JM, Topiwala MA, et al. Prefrontal entrainment of amygdala activity signals safety in learned fear and innate anxiety[J]. Nat Neurosci, 2014, 17(1): 106–13. doi: 10.1038/nn.3582
[11]	Schier CJ, Mangieri RA, Dilly GA, et al. Microdialysis of ethanol during operant ethanol self-administration and ethanol determination by gas chromatography[J]. J Vis Exp, 2012, (67): e4142.
[12]	You R, Liu Y, Chang RC. A Behavioral Test Battery for the Repeated Assessment of Motor Skills, Mood, and Cognition in Mice[J]. J Vis Exp, 2019, (145).
[13]	Deacon RM. Measuring motor coordination in mice[J]. J Vis Exp, 2013, (75): e2609. https://pubmed.ncbi.nlm.nih.gov/23748408/
[14]	Yankelevitch-Yahav R, Franko M, Huly A, et al. The forced swim test as a model of depressive-like behavior[J]. J Vis Exp, 2015, (97).
[15]	Marcinkiewcz CA, Dorrier CE, Lopez AJ, et al. Ethanol induced adaptations in 5-HT2c receptor signaling in the bed nucleus of the stria terminalis: implications for anxiety during ethanol withdrawal[J]. Neuropharmacology, 2015, 89: 157–67. doi: 10.1016/j.neuropharm.2014.09.003
[16]	Kraeuter AK, Guest PC, Sarnyai Z. The Open Field Test for Measuring Locomotor Activity and Anxiety-Like Behavior[J]. Methods Mol Biol, 2019, 1916: 99–103. https://pubmed.ncbi.nlm.nih.gov/30535687/
[17]	Carriel V, Campos A, Alaminos M, et al. Staining Methods for Normal and Regenerative Myelin in the Nervous System[J]. Methods Mol Biol, 2017, 1560: 207–218. https://pubmed.ncbi.nlm.nih.gov/28155156/
[18]	Wang J, Jiang L, Du H, et al. An ethanol vapor chamber system for small animals[J]. J Neurosci Methods, 2012, 208(1): 79–85. doi: 10.1016/j.jneumeth.2012.04.017
[19]	Ceccarini J, Leurquin-Sterk G, Crunelle CL, et al. Recovery of Decreased Metabotropic Glutamate Receptor 5 Availability in Abstinent Alcohol-Dependent Patients[J]. J Nucl Med, 2020, 61(2): 256–262. doi: 10.2967/jnumed.119.228825
[20]	Gilpin NW, Richardson HN, Cole M, et al. Vapor inhalation of alcohol in rats[J]. Curr Protoc Neurosci, 2008, Chapter 9: Unit-9.29.
[21]	Fu R, Mei Q, Shiwalkar N, et al. Anxiety during alcohol withdrawal involves 5-HT2C receptors and M-channels in the lateral habenula[J]. Neuropharmacology, 2020, 163: 107863. doi: 10.1016/j.neuropharm.2019.107863
[22]	Blokland A, Ten Oever S, van Gorp D, et al. The use of a test battery assessing affective behavior in rats: order effects[J]. Behav Brain Res, 2012, 228(1): 16–21. doi: 10.1016/j.bbr.2011.11.042
[23]	Faulkner ML, Momenan R, Leggio L. A neuroimaging investigation into the role of peripheral metabolic biomarkers in the anticipation of reward in alcohol use[J]. Drug Alcohol Depend, 2021, 221: 108638. doi: 10.1016/j.drugalcdep.2021.108638
[24]	Seibenhener ML, Wooten MC. Use of the Open Field Maze to measure locomotor and anxiety-like behavior in mice[J]. J Vis Exp, 2015, (96): e52434. doi: 10.3791/52434
[25]	Ma T, Cheng Y, Roltsch Hellard E, et al. Bidirectional and long-lasting control of alcohol-seeking behavior by corticostriatal LTP and LTD[J]. Nat Neurosci, 2018, 21(3): 373–383. doi: 10.1038/s41593-018-0081-9
[26]	Ma T, Huang Z, Xie X, et al. Chronic alcohol drinking persistently suppresses thalamostriatal excitation of cholinergic neurons to impair cognitive flexibility[J]. J Clin Invest, 2022, 132(4).
[27]	Padilla-Coreano N, Bolkan SS, Pierce GM, et al. Direct Ventral Hippocampal-Prefrontal Input Is Required for Anxiety-Related Neural Activity and Behavior[J]. Neuron, 2016, 89(4): 857–66. doi: 10.1016/j.neuron.2016.01.011
[28]	Adhikari A, Topiwala MA, Gordon JA. Synchronized activity between the ventral hippocampus and the medial prefrontal cortex during anxiety[J]. Neuron, 2010, 65(2): 257–69. doi: 10.1016/j.neuron.2009.12.002
[29]	Holmes A, Fitzgerald PJ, MacPherson KP, et al. Chronic alcohol remodels prefrontal neurons and disrupts NMDAR-mediated fear extinction encoding[J]. Nat Neurosci, 2012, 15(10): 1359–61. doi: 10.1038/nn.3204
[30]	Frost ME, Peterson VL, Bird CW, et al. Effects of Ethanol Exposure and Withdrawal on Neuronal Morphology in the Agranular Insular and Prelimbic Cortices: Relationship with Withdrawal-Related Structural Plasticity in the Nucleus Accumbens[J]. Brain Sci, 2019, 9(8): 180.
[31]	Roerecke M. Alcohol's Impact on the Cardiovascular System[J]. Nutrients, 2021, 13(10): 3419.