A Novel long non-coding RNA NFIA-AS1 is down-regulated in gastric cancer and inhibits proliferation of gastric cancer cells
- Available Online: 2019-05-20
Abstract: Gastric cancer (GC)is one of the most common malignant gastrointestinal tumors, and its morbidity and mortality account for the second and third place respectively in malignant tumors in China. As a major participant in tumor biology, the abnormal expression of long non-coding RNA (lncRNAs) in cancer cells is closely related to the occurrence and development of tumors and plays the role of oncogenes or tumor suppressor genes.In this study, we identified a novel lncRNA NFIA-AS1 and explored its role and clinical significance in gastric cancer. Real-time quantitative PCR was performed to detect the expression of NFIA-AS1 in tumor tissues andcorresponding normal tissues from 42 pairs of GC samples. The lower expression of NFIA-AS1 wassignificantly associated with larger tumor size, lower histologicalgrade, and advanced TNM stage. Kaplan-meier analysisshowed that NFIA-AS1 expression couldbe used as anindependent predictor of overall survival.We alsodemonstrated that overexpression of NFIA-AS1significantly inhibited the proliferation of gastric cancer cells through affecting p16 levels. In conclusion, our results suggest that the lncRNA NFIA-AS1 may play the role of tumor suppressor gene, and serve asa biomarker for prognosis or progression of gastric cancer.