• ISSN 16748301
  • CN 32-1810/R
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Citation:

Effect of methotrexate on collagen-induced arthritis in male Wistar rats

  • Received Date: 2017-02-28
    Accepted Date: 2017-04-08

    Fund Project: This is an open access article under the Creative Commons Attribution (CC BY 4.0) license, which permits others to distribute, remix, adapt and build upon this work, for commercial use, provided the original work is properly cited

  • To evaluate the effect of methotrexate on collagen-induced arthritis, micro-computed tomography (micro-CT) and histopathological analyses were used in male Wistar rats. Rats were divided randomly into three groups. Group 1 was treated with 0.9% saline, and groups 2 and 3 were boosted with type Ⅱ collagen. From day 21 to 42, groups 1 and 2 were orally treated with 0.9% saline and group 3 was orally treated with 1.5 mg/kg methotrexate. All rats were sacrificed at day 42 after the first collagen treatment. Micro-CT analyses showed bony parameters, such as bone volume and trabecular number, were decreased in group 2 compared to group 1, and these parameters were recovered in group 3. Histopathological examination and pathological parameter scoring showed that the knee joints of rats in group 2 had severe joint destruction, showing cartilage and bone erosion, enlarged cavities with inflammatory cell infiltration and activation of synovial fibroblasts. By contrast, these changes were reduced in group 3. Taken together, methotrexate treatment showed therapeutic potential in male rat collagen-induced arthritis model, and micro-CT analysis and histopathological tools could be integrated to assess the quantification/qualification of arthritic lesions.
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  • [1] Sandell LJ, Aigner T. Articular cartilage and changes in arthritis. An introduction: cell biology of osteoarthritis[J]. Arthritis Res, 2001, 3(2): 107-113.
    [2] Otero M, Goldring MB. Cells of the synovium in rheumatoid arthritis. Chondrocytes[J]. Arthritis Res Ther, 2007, 9(5): 220.
    [3] Scott DL, Wolfe F, Huizinga TW. Rheumatoid arthritis[J]. Lancet, 2010, 376(9746): 1094-1108.
    [4] Scott DL. Biologics-based therapy for the treatment of rheumatoid arthritis[J]. Clin Pharmacol Ther, 2012, 91(1): 30-43.
    [5] Brand DD, Latham KA, Rosloniec EF. Collagen-induced arthritis[J]. Nat Protoc, 2007, 2(5): 1269-1275.
    [6] Myers LK, Rosloniec EF, Cremer MA, et al. Collagen-induce arthritis, an animal model of autoimmunity[J]. Life Sci, 1997, 61(19): 1861-1878.
    [7] Maruotti N, Cantatore FP, Crivellato E, et al. Macrophages in rheumatoid arthritis[J]. Histol Histopathol, 2007, 22(5): 581- 586.
    [8] Bevaart L, Vervoordeldonk MJ, Tak PP. Evaluation of therapeutic targets in animal models of arthritis: how does it relate to rheumatoid arthritis?[J]. Arthritis Rheum, 2010, 62(8): 2192-2205.
    [9] Carvalheiro H, da Silva JA, Souto-Carneiro MM. Potential roles for CD8(+) T cells in rheumatoid arthritis[J]. Autoimmun Rev, 2013, 12(3): 401-409.
    [10] Conway JR, Carragher NO, Timpson P. Developments in preclinical cancer imaging: innovating the discovery of therapeutics[J]. Nat Rev Cancer, 2014, 14(5): 314-328.
    [11] de Jong M, Essers J, van Weerden WM. Imaging preclinical tumour models: improving translational power[J]. Nat Rev Cancer, 2014, 14(7): 481-493.
    [12] Schambach SJ, Bag S, Schilling L, et al. Application of microCT in small animal imaging[J]. Methods, 2010, 50(1): 2-13.
    [13] Koba W, Jelicks LA, Fine EJ. MicroPET/SPECT/CT imaging of small animal models of disease[J]. Am J Pathol, 2013, 182(2): 319-324.
    [14] Kim YH, Kang JS. Effect of methotrexate on collagen-induced arthritis assessed by micro-computed tomography and histopathological examination in female rats[J]. Biomol Ther (Seoul), 2015, 23(2): 195-200.
    [15] Knoerzer DB, Donovan MG, Schwartz BD, et al. Clinical and histological assessment of collagen-induced arthritis progression in the diabetes-resistant BB/Wor rat[J]. Toxicol Pathol, 1997, 25(1): 13-19.
    [16] Gerwin N, Bendele AM, Glasson S, et al. The OARSI histopathology initiative- recommendations for histological assessments of osteoarthritis in the rat[J]. Osteoarthritis Cartilage, 2010, 18(S3): S24-34.
    [17] Batiste DL, Kirkley A, Laverty S, et al. Ex vivo characterization of articular cartilage and bone lesions in a rabbit ACL transection model of osteoarthritis using MRI and micro-CT[J]. Osteoarthritis Cartilage, 2004, 12(12): 986-996.
    [18] Ishikawa T, Nishigaki F, Miyata S, et al. Prevention of progressive joint destruction in collagen-induced arthritis in rats by a novel matrix metalloproteinase inhibitor, FR255031[J]. Br J Pharmacol, 2005, 144(1): 133-143.
    [19] Peterson JD, Labranche TP, Vasquez KO, et al. Optical tomographic imaging discriminates between disease-modifying anti-rheumatic drug (DMARD) and non-DMARD efficacy in collagen antibody-induced arthritis[J]. Arthritis Res Ther, 2010, 12(3): R105.
    [20] Ryu JH, Lee A, Chu JU, et al. Early diagnosis of arthritis in mice with collagen-induced arthritis, using a fluorogenic matrix metalloproteinase 3-specific polymeric probe[J]. Arthritis Rheum, 2011, 63(12): 3824-3832.
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Effect of methotrexate on collagen-induced arthritis in male Wistar rats

  • Department of Biomedical Laboratory Science, Namseoul University, Cheonan 31020, Republic of Korea
Fund Project:  This is an open access article under the Creative Commons Attribution (CC BY 4.0) license, which permits others to distribute, remix, adapt and build upon this work, for commercial use, provided the original work is properly cited

Abstract: To evaluate the effect of methotrexate on collagen-induced arthritis, micro-computed tomography (micro-CT) and histopathological analyses were used in male Wistar rats. Rats were divided randomly into three groups. Group 1 was treated with 0.9% saline, and groups 2 and 3 were boosted with type Ⅱ collagen. From day 21 to 42, groups 1 and 2 were orally treated with 0.9% saline and group 3 was orally treated with 1.5 mg/kg methotrexate. All rats were sacrificed at day 42 after the first collagen treatment. Micro-CT analyses showed bony parameters, such as bone volume and trabecular number, were decreased in group 2 compared to group 1, and these parameters were recovered in group 3. Histopathological examination and pathological parameter scoring showed that the knee joints of rats in group 2 had severe joint destruction, showing cartilage and bone erosion, enlarged cavities with inflammatory cell infiltration and activation of synovial fibroblasts. By contrast, these changes were reduced in group 3. Taken together, methotrexate treatment showed therapeutic potential in male rat collagen-induced arthritis model, and micro-CT analysis and histopathological tools could be integrated to assess the quantification/qualification of arthritic lesions.

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