• ISSN 16748301
  • CN 32-1810/R
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Citation:

Thiel-embalming technique: investigation of possible modification in embalming tissue as evaluation model for radiofrequency ablation

  • Received Date: 2016-11-09
    Accepted Date: 2017-01-08

    Fund Project: The authors thank Mr. Donald McLean for his help in experimental support, Dr. Roos Eisma, Miss Amanda Hunter and Miss. Seaneen McDougall, Dr. Daniel Melling, Dr. Lingxi Zhao and Miss Radoslava Yanakeiva for fruitful discussions. L. P. Y. thanks China Scholar Council, for enabling him to study abroad and financially supporting in the past nine months.

  • Contrary to freezing preservation and formalin embalming, Thiel embalmed cadaver presents soft texture and color very close to that of living organism, and many applications based on Thiel embalmed cadavers have been reported. However, Thiel embalmed cadavers cannot be used as reliable evaluation model for radiofrequency ablation (RFA) due to dramatic changes of electrical conductivity in the embalmed tissue. To address this issue, we investigated various modifications of the original Thiel embalming solution. By altering the chemicals' species and concentration we figured out a formula that can greatly reduce the embalming fluid's electrical conductivity without significantly compromising the 18-day embalmed kidney samples' suppleness and color. We also investigated a two-stage embalming technique by first submerging the kidney sample into original Thiel's tank fluid for 28 days, then the sample was withdrawn from the tank fluid and placed into modified dilution fluids for additional two weeks. Stiffening and discoloration occurred in these diluted samples implying the reversibility of Thiel-embalmed tissues' suppleness and color with the removal of the strong electrolytes. This study presents a modified embalming method which could be used for RFA evaluation and also helps our understanding of the mechanism of embalmment process.
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Thiel-embalming technique: investigation of possible modification in embalming tissue as evaluation model for radiofrequency ablation

  • 1 Institute for Medical Science and Technology, University of Dundee, Dundee, Scotland DD2 1FD, United Kingdom;
  • 2 School of Engineering, Physics and Mathematics, University of Dundee, Dundee, Scotland DD1 4HN, United Kingdom;
  • 3 College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410000, China;
  • 4 College of Engineering, Peking University, Beijing 100671, China
Fund Project:  The authors thank Mr. Donald McLean for his help in experimental support, Dr. Roos Eisma, Miss Amanda Hunter and Miss. Seaneen McDougall, Dr. Daniel Melling, Dr. Lingxi Zhao and Miss Radoslava Yanakeiva for fruitful discussions. L. P. Y. thanks China Scholar Council, for enabling him to study abroad and financially supporting in the past nine months.

Abstract: Contrary to freezing preservation and formalin embalming, Thiel embalmed cadaver presents soft texture and color very close to that of living organism, and many applications based on Thiel embalmed cadavers have been reported. However, Thiel embalmed cadavers cannot be used as reliable evaluation model for radiofrequency ablation (RFA) due to dramatic changes of electrical conductivity in the embalmed tissue. To address this issue, we investigated various modifications of the original Thiel embalming solution. By altering the chemicals' species and concentration we figured out a formula that can greatly reduce the embalming fluid's electrical conductivity without significantly compromising the 18-day embalmed kidney samples' suppleness and color. We also investigated a two-stage embalming technique by first submerging the kidney sample into original Thiel's tank fluid for 28 days, then the sample was withdrawn from the tank fluid and placed into modified dilution fluids for additional two weeks. Stiffening and discoloration occurred in these diluted samples implying the reversibility of Thiel-embalmed tissues' suppleness and color with the removal of the strong electrolytes. This study presents a modified embalming method which could be used for RFA evaluation and also helps our understanding of the mechanism of embalmment process.

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