• ISSN 16748301
  • CN 32-1810/R
Volume 32 Issue 3
Mar.  2018
Article Contents

Citation:

Tcf7l1 promotes transcription of Kruppel-like factor 4 during Xenopus embryogenesis

  • Received Date: 2017-03-23
    Accepted Date: 2017-06-16

    Fund Project: We thank Model Animal Research Center of Nanjing University for their assistance in data collection. This work was supported by the Start-up Funding of Henan University of Science and Technology (13480027) to Q. C. and the Key Science Foundation of Nanjing Medical University(2015NJMUZD002) and the Natural Science Foundation of Higher Education Institutions of Jiangsu Province (16KJB-180020) and Natural Science Foundation of Jiangsu Province (BK20171053) and National Natural Science Funds of China (81702747) to C.L.

  • Kruppel-like factor 4 (Klf4) is a zinc finger transcription factor and plays crucial roles in Xenopus embryogenesis. However, its regulation during embryogenesis is still unclear. Here, we report that Tcf7l1, a key downstream transducer of the Wnt signaling pathway, could promote Klf4 transcription and stimulate Klf4 promoter activity in early Xenopus embryos. Furthermore, cycloheximide treatment showed a direct effect on Klf4 transcription facilitated by Tcf7l1. Moreover, the dominant negative form of Tcf7l1 (dnTcf7l1), which lacks N-terminus of the β-catenin binding motif, could still activate Klf4 transcription, suggesting that this regulation is Wnt/β-catenin independent. Taken together, our results demonstrate that Tcf7l1 lies upstream of Klf4 to maintain its expression level during Xenopus embryogenesis.
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Tcf7l1 promotes transcription of Kruppel-like factor 4 during Xenopus embryogenesis

  • 1. College of Medicine, Henan University of Science and Technology, Luoyang, Henan 471023, China
  • 2. Department of Developmental Genetics, Nanjing Medical University, Nanjing, Jiangsu 211166, China
Fund Project:  We thank Model Animal Research Center of Nanjing University for their assistance in data collection. This work was supported by the Start-up Funding of Henan University of Science and Technology (13480027) to Q. C. and the Key Science Foundation of Nanjing Medical University(2015NJMUZD002) and the Natural Science Foundation of Higher Education Institutions of Jiangsu Province (16KJB-180020) and Natural Science Foundation of Jiangsu Province (BK20171053) and National Natural Science Funds of China (81702747) to C.L.

Abstract: Kruppel-like factor 4 (Klf4) is a zinc finger transcription factor and plays crucial roles in Xenopus embryogenesis. However, its regulation during embryogenesis is still unclear. Here, we report that Tcf7l1, a key downstream transducer of the Wnt signaling pathway, could promote Klf4 transcription and stimulate Klf4 promoter activity in early Xenopus embryos. Furthermore, cycloheximide treatment showed a direct effect on Klf4 transcription facilitated by Tcf7l1. Moreover, the dominant negative form of Tcf7l1 (dnTcf7l1), which lacks N-terminus of the β-catenin binding motif, could still activate Klf4 transcription, suggesting that this regulation is Wnt/β-catenin independent. Taken together, our results demonstrate that Tcf7l1 lies upstream of Klf4 to maintain its expression level during Xenopus embryogenesis.

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