• ISSN 16748301
  • CN 32-1810/R
Volume 25 Issue 2
Feb.  2011
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Citation:

UVB suppresses PTEN expression by upregulating miR-141 in HaCaT cells

  • Fund Project: This study was supported by the National Natural Science Foundation of China (No. 30771946 and No. 81000700).

  • MicroRNAs (miRNAs) are 21 to 24 nucleotide, non-coding RNA molecules that post-transcriptionally regulate the expression of target genes. Ultraviolet B (UVB) radiation has been shown to inhibit phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression in HaCaT cells through an unknown mechanism. In this study, we investigated whether miR-141 can regulate UVB exposure-mediated inhibition of PTEN expression. Real-time RT-PCR, annexin V/fluorescein isothiocyanate staining, Western blotting and anti-miRNA oligonucle-otide transfection were employed in this study. We found that upregulation of miR-141 expression after UVB ir-radiation was inversely correlated with PTEN expression levels in HaCaT cells. Furthermore, miR-141 expression increased apoptosis, while anti-miR-141 partly restored PTEN expression and reversed the pro-apoptosis effect of UVB. UVB suppresses the expression of PTEN by upregulating miR-141 in HaCaT cells. Therefore, miR-141 is a potential gene therapy target for UVB-induced photodamage.
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UVB suppresses PTEN expression by upregulating miR-141 in HaCaT cells

  • 1. Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiansu 210029, China
Fund Project:  This study was supported by the National Natural Science Foundation of China (No. 30771946 and No. 81000700).

Abstract: MicroRNAs (miRNAs) are 21 to 24 nucleotide, non-coding RNA molecules that post-transcriptionally regulate the expression of target genes. Ultraviolet B (UVB) radiation has been shown to inhibit phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression in HaCaT cells through an unknown mechanism. In this study, we investigated whether miR-141 can regulate UVB exposure-mediated inhibition of PTEN expression. Real-time RT-PCR, annexin V/fluorescein isothiocyanate staining, Western blotting and anti-miRNA oligonucle-otide transfection were employed in this study. We found that upregulation of miR-141 expression after UVB ir-radiation was inversely correlated with PTEN expression levels in HaCaT cells. Furthermore, miR-141 expression increased apoptosis, while anti-miR-141 partly restored PTEN expression and reversed the pro-apoptosis effect of UVB. UVB suppresses the expression of PTEN by upregulating miR-141 in HaCaT cells. Therefore, miR-141 is a potential gene therapy target for UVB-induced photodamage.

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