• ISSN 1674-8301
  • CN 32-1810/R
Volume 26 Issue 1
Jan.  2012
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Joshua Muli Mutiso, John Chege Macharia, Evans Taracha, Michael Muita Gicheru. Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)[J]. The Journal of Biomedical Research, 2012, 26(1): 8-16. doi: 10.1016/S1674-8301(12)60002-5
Citation: Joshua Muli Mutiso, John Chege Macharia, Evans Taracha, Michael Muita Gicheru. Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)[J]. The Journal of Biomedical Research, 2012, 26(1): 8-16. doi: 10.1016/S1674-8301(12)60002-5

Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)

doi: 10.1016/S1674-8301(12)60002-5
Funds:

This work was supported by a grant from the National Council for Science and Technology, Government of Kenya (No. NCST 51003 CALL2 226)

  • Received Date: 2011-07-23
  • Publish Date: 2012-03-05
  • In a previous immunogenicity and efficacy study in mice, montanide ISA 720 (MISA) was indicated to be a better adjuvant than bacillus calmette guerin vaccine (BCG) for a Leishmania vaccine. In the present study, we report the safety, immunogenicity and efficacy of Leishmania donovani (L. donovani) sonicated antigen delivered with alum-BCG (AlBCG), MISA or monophosphoryl lipid A (MPLA) in vervet monkeys following intradermal inoculums. Vaccinated and control animals were challenged with virulent L. donovani parasites and the parasitic burden was determined. Only animals vaccinated with alum-BCG adversely reacted to the inoculum by produc-ing ulcerative erythematous skin indurations. Non-parametric ANOVA followed by a post test showed signifi-cantly higher IgG antibodies, and revealed the presence of lymphoproliferative and interferon gamma responses in both AlBCG+Ag and MISA+Ag as compared to the MPLA+Ag or other groups (P < 0.001). We conclude that L. donovani sonicated antigen containing MISA is safe and is associated with protective immune response against Leishmania donovani infection in the vervet monkey model.
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Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)

doi: 10.1016/S1674-8301(12)60002-5
Funds:

This work was supported by a grant from the National Council for Science and Technology, Government of Kenya (No. NCST 51003 CALL2 226)

Abstract: In a previous immunogenicity and efficacy study in mice, montanide ISA 720 (MISA) was indicated to be a better adjuvant than bacillus calmette guerin vaccine (BCG) for a Leishmania vaccine. In the present study, we report the safety, immunogenicity and efficacy of Leishmania donovani (L. donovani) sonicated antigen delivered with alum-BCG (AlBCG), MISA or monophosphoryl lipid A (MPLA) in vervet monkeys following intradermal inoculums. Vaccinated and control animals were challenged with virulent L. donovani parasites and the parasitic burden was determined. Only animals vaccinated with alum-BCG adversely reacted to the inoculum by produc-ing ulcerative erythematous skin indurations. Non-parametric ANOVA followed by a post test showed signifi-cantly higher IgG antibodies, and revealed the presence of lymphoproliferative and interferon gamma responses in both AlBCG+Ag and MISA+Ag as compared to the MPLA+Ag or other groups (P < 0.001). We conclude that L. donovani sonicated antigen containing MISA is safe and is associated with protective immune response against Leishmania donovani infection in the vervet monkey model.

Joshua Muli Mutiso, John Chege Macharia, Evans Taracha, Michael Muita Gicheru. Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)[J]. The Journal of Biomedical Research, 2012, 26(1): 8-16. doi: 10.1016/S1674-8301(12)60002-5
Citation: Joshua Muli Mutiso, John Chege Macharia, Evans Taracha, Michael Muita Gicheru. Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)[J]. The Journal of Biomedical Research, 2012, 26(1): 8-16. doi: 10.1016/S1674-8301(12)60002-5

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