TSP-1 promotes glomerular mesangial cell proliferation and extracellular matrix secretion in Thy-1 nephritis rats
The study was supported by grants from National Natural Science Foundations of China (No. 31000396, and No.81072402) and grants from Natural Science Foundations of Jiangsu Province in China (No. BK2009417, No. 10KJB310006, and No. 09hx43).
- Thy-1 nephritis /
- glomerular mesangial cells (GMCs) /
- proliferation /
- extracellular matrix /
- throm-bospondin-1 (TSP-1)
Abstract: The proliferation of glomerular mesangial cells (GMC) and secretion of the extracellular matrix (ECM) in rat with Thy-1 nephritis (Thy-1N) resembling human mesangioproliferative glomerulonephritis have been explored for many years; however, the molecular mechanisms of GMC proliferation and ECM production remain unclear. Our previous studies have demonstrated that the thrombospondin-1 (TSP-1) gene was involved in mediating rat GMC proliferation and ECM synthesis induced by sublytic C5b-9 in vitro. In the present study, the roles of the TSP-1 gene in GMC proliferation, ECM production, and urinary protein secretion in Thy-1N rats were determined by using TSP-1 small hairpin RNA, and the results revealed that silencing of the TSP-1 gene in rat renal tissues could diminish GMC proliferation (P < 0.01) and ECM secretion (P < 0.01) as well as urinary protein secretion (P < 0.05) in Thy-1N rats. Together, the current findings suggested that TSP-1 gene expression was required for GMC proliferation and ECM production in Thy-1N rats.
|Citation:||Wen Qiu, Yan Li, Jianbo Zhou, Chenhui Zhao, Jing Zhang, Kai Shan, Dan Zhao, Yingwei Wang. TSP-1 promotes glomerular mesangial cell proliferation and extracellular matrix secretion in Thy-1 nephritis rats[J]. The Journal of Biomedical Research, 2011, 25(6): 402-410. doi: 10.1016/S1674-8301(11)60053-5|