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Kaibo Lin,Shikun Zhang,Jieli Chen,Ding Yang,Mengyi Zhu,Eugene Yujun Xu.Journal of Biomedical Research,2018,32(6):434-441
Generation and functional characterization of a conditional Pumilio2 null allele
Received:November 07, 2017  Revised:November 28, 2017
DOI10.7555/JBR.32.20170117
KeywordsRNA-binding protein, Pumilio2, PUF, fertility, viability
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Kaibo Lin State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu , China.
Shikun Zhang State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu , China.
Jieli Chen State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu , China.
Ding Yang State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu , China.
Mengyi Zhu State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu , China.
Eugene Yujun Xu State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu , China.
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Abstract
      The highly conserved RNA binding protein PUF (Pumilio/FBF) family is present throughout eukaryotes from yeast to mammals, with critical roles in development, fertility and the nervous system. However, the function of the mammalian PUF family members remains underexplored. Our previous study reported that a gene-trap mutation of Pum2 results in a smaller testis but does not impact fertility and viability. Although the gene-trap mutation disrupted the key functional domain of PUM proteinCPUM-HD (Pumilio homology domain), but still produced a chimeric Pum2-β-geo protein containing part of PUM2, raising a question if such a chimeric protein may provide any residual function or contribute to the reproductive phenotype. Here, we report the generation of a conditional PUM2 allele, when knocked out, producing no residual PUM2 and hence a complete loss-of-function allele. We also uncovered small but significant reduction of male fertility and viability in the mutants, suggesting requirement of PUM2 for male fertility and viability
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