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Lixing Wu,Yuyu Xu,Zhijian Yang,Qing Feng.Journal of Biomedical Research,2018,32(5):371-379
Hydroxytyrosol and olive leaf extract exert cardioprotective effects by inhibiting GRP78 and CHOP expression
Received:November 01, 2017  Revised:January 24, 2018
DOI£º10.7555/JBR.32.20170111
Keywords£ºmyocardial infarction, olive leaf extract, hydroxytyrosol, endoplasmic reticulum stress
Grant Program£º
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AuthorInstitution
Lixing Wu Department of Nutrition and Food Safety, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu , China
Yuyu Xu Department of Nutrition and Food Safety, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu , China
Zhijian Yang Department of Cardiology, Jiangsu Province Hospital, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu , China.
Qing Feng Department of Nutrition and Food Safety, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu , China
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Abstract £º
      Myocardial infarction triggers massive biochemical changes, even cardiac cell death. Endoplasmic reticulum stress is involved in the pathology of myocardial infarction-mediated apoptosis. In the present study, myocardial cell line H9c2 cells were treated with cobalt chloride (CoCl2) to induce hypoxia. Isoproterenol was used for two successive days to induce myocardial infarction in SD rats. The cardioprotective effect of olive leaf extract (OLE) and its main constituent hydroxytyrosol and the underlying mechanisms were evaluated. The results showed that hydroxytyrosol markedly protected H9c2 cells against CoCl2-induced apoptosis. Hydroxytyrosol could reduce the mRNA and protein expression of GRP78 and CHOP induced by CoCl2 in vitro. In vivo, the decreased ejection fraction and fractional shortening, increased heart weight/body ratio, the formation of infarction, disordered cardiac muscle fibers and infiltration of inflammatory cells induced by isoproterenol could be significantly ameliorated by pretreatment with OLE for a month. Similarly, OLE could also reverse the increase of GRP78 and CHOP expression induced by isoproterenol. Therefore, OLE and hydroxytyrosol exert a cardioprotective effect through endoplasmic reticulum stress, which could be a new target for the prevention and treatment of cardiovascular diseases.
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