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Qing Cao,Yan Shen,Wei Zheng,Hao Liu,Chen Liu.Journal of Biomedical Research,2018,32(3):215-221
Tcf7l1 promotes transcription of Kruppel-like factor 4 during Xenopus embryogenesis
Received:March 23, 2017  Revised:June 16, 2017
DOI10.7555/JBR.32.20170056
KeywordsKruppel-like factor 4 (Klf4), Tcf7l1, transcription regulation, Xenopus laevis
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AuthorInstitution
Qing Cao College of Medicine, Henan University of Science and Technology, Luoyang, Henan , China
Yan Shen College of Medicine, Henan University of Science and Technology, Luoyang, Henan , China
Wei Zheng College of Medicine, Henan University of Science and Technology, Luoyang, Henan , China
Hao Liu College of Medicine, Henan University of Science and Technology, Luoyang, Henan , China
Chen Liu Department of Developmental Genetics, Nanjing Medical University, Nanjing, Jiangsu , China
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Abstract
      Kruppel-like factor 4 (Klf4) is a zinc finger transcription factor and plays crucial roles in Xenopus embryogenesis. However, its regulation during embryogenesis is still unclear. Here, we report that Tcf7l1, a key downstream transducer of the Wnt signaling pathway, could promote Klf4 transcription and stimulate Klf4 promoter activity in early Xenopus embryos. Furthermore, cycloheximide treatment showed a direct effect on Klf4 transcription facilitated by Tcf7l1. Moreover, the dominant negative form of Tcf7l1 (dnTcf7l1), which lacks N-terminus of the β-catenin binding motif, could still activate Klf4 transcription, suggesting that this regulation is Wnt/β-catenin independent. Taken together, our results demonstrate that Tcf7l1 lies upstream of Klf4 to maintain its expression level during Xenopus embryogenesis.
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